DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHO TOMETRIC AND RP-HPLC METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUI NONE, HYDROCORTISONE AND TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION
By: Marwa, AlJamal K.
Contributor(s): Gazy, Azza A.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(11).Description: 10-16p.Subject(s): PHARMACEUTICSOnline resources: Click here In: International journal of pharmacy and pharmaceutical scienceSummary: Objective: Development and validation of spectrophotometric an d RP-HPLC methods for the simultaneous determination of Hydroquinone (HQ), Hydrocortisone (HC) and Tretinoin (TRT) ternary combi nation in pharmaceutical preparation. Methods: The proposed spectrophotometric method was able to determine TRT directly from its absorption spectrum at 362 nm, however, HQ and HC from their first derivative spectra at 284 nm and 252 nm, respectively, without any separation step. The RP-HPLC method was developed using a C 18 Sunfire © waters column with a mobile phase composed of acet onitrile: phosphate buffer (adjusted to pH 6.1 using ortho-phosphoric acid) in the ratio of 30:70 %, v/v, respectively at a flow rate of 0.8 ml/min. Quantification was based on measurin g peak areas at 260 nm. Results: The spectrophotometric method was able to selective ly quantify each of HQ, HC and TRT in the ranges of 10-50 μg/ml, 2-10 μg/ml and 0.5-5 μg/ml, respectively. The RP-HPLC method was able to produc e well-resolved peaks after 3.0, 8.2 and 20.2 min, in the ranges of 2-10 μg/ml, 0.1-1 μg/ml and 0.05-2 μg/ml, for HQ, HC and TRT, respectively. The obtain ed A, D 1 or peak areas values plotted against the concentra tion of each of the three components showed linear response in the stated ranges. Both methods were validated in terms of linearity, LOD, LOQ, pre cision, accuracy and selectivity. Conclusion: Both developed proposed methods were applied for th e determination of the active ingredients in the ph armaceutical formulation and the common excipients did not interfere in the anal ysis. The RP-HPLC method proved to be more sensitiv e when compared to the applied spectrophotometric method. However, the applied spe ctrophotometric methods, considered as green analyt ical chemistry, is a simple, time-saving method that requires minimal use of a hazardous sol vent.Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|
Articles Abstract Database | School of Pharmacy Archieval Section | Not for loan | 2020850 |
Objective:
Development and validation of spectrophotometric an
d RP-HPLC methods for the simultaneous determination
of Hydroquinone (HQ),
Hydrocortisone (HC) and Tretinoin (TRT) ternary combi
nation in pharmaceutical preparation.
Methods:
The proposed spectrophotometric method was able to
determine TRT directly from its absorption spectrum
at 362 nm, however, HQ and
HC from their first derivative spectra at 284 nm and
252 nm, respectively, without any separation step.
The RP-HPLC method was developed using a
C
18
Sunfire
©
waters column with a mobile phase composed of acet
onitrile: phosphate buffer (adjusted to pH 6.1 using
ortho-phosphoric acid) in the
ratio of 30:70 %, v/v, respectively at a flow rate
of 0.8 ml/min. Quantification was based on measurin
g peak areas at 260 nm.
Results:
The spectrophotometric method was able to selective
ly quantify each of HQ, HC and TRT in the ranges of
10-50 μg/ml, 2-10 μg/ml and 0.5-5 μg/ml,
respectively. The RP-HPLC method was able to produc
e well-resolved peaks after 3.0, 8.2 and 20.2 min,
in the ranges of 2-10 μg/ml, 0.1-1 μg/ml and 0.05-2
μg/ml, for HQ, HC and TRT, respectively. The obtain
ed A, D
1
or peak areas values plotted against the concentra
tion of each of the three components showed
linear response in the stated ranges. Both methods
were validated in terms of linearity, LOD, LOQ, pre
cision, accuracy and selectivity.
Conclusion:
Both developed proposed methods were applied for th
e determination of the active ingredients in the ph
armaceutical formulation and
the common excipients did not interfere in the anal
ysis. The RP-HPLC method proved to be more sensitiv
e when compared to the applied
spectrophotometric method. However, the applied spe
ctrophotometric methods, considered as green analyt
ical chemistry, is a simple, time-saving
method that requires minimal use of a hazardous sol
vent.
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